Scientific Program

Conference Series LLC Ltd invites all the participants across the globe to attend 5th International Conference on Parasitology & Microbiology Paris, France.

Day 2 :

Parasitology 2018 International Conference Keynote Speaker Julio V Figueroa photo
Biography:

Julio V Figueroa is currently working as a Researcher and Head of the National Research Center for Veterinary Parasitology (CENID-PAVET) in Jiutepec, Morelos, Mexico. He obtained his Veterinary Medicine degree from the State of Mexico Autonomous University in Toluca, Mexico, and the MSc and PhD degrees in Veterinary Pathology and Microbiology at the University of Columbia-Missouri, in Columbia, MO, USA. He has conducted research on tick borne diseases of cattle during the past 30 years and has published over 70 research papers in peer reviewed international journals

Abstract:

Merozoite Surface Antigen-1 (MSA-1) and Ropthry Associated Protein-1 (RAP-1) for Babesia bovis and Babesia bigemina, respectively, have been involved in the invasion to the host erythrocyte. Moreover, considered as highly immunogenic proteins, they are candidate proteins to be used as specific antigens in Bovine babesiosis diagnosis. The main objective of this work was to determine the antibody recognition of Babesia bovis MSA-1 and RAP-1 antigens in serum of sheep immunized with the corresponding recombinant proteins, by using the Indirect Fluorescent Antibody Test (IFAT) and an indirect Enzyme-Linked Immunosorbent Assay (iELISA). The results of the study showed that recognition for the native antigen and the recombinant antigens of Babesia bovis and Babesia bigemina took place after the second immunization of animals as assessed by the two serological assays. The IFAT antibody titers for sheep inoculated with MSA-1 were 1:5120-1:10240, whereas for sheep inoculated with RAP-1 titers of 1:640 and 1:1280 were determined. A maximum absorbance reading of 0.689 and 0.600 were determined for MSA-1 and RAP-1, respectively, in the iELISA technique, concluding that the specific B. bovis MSA-1 and B. bigemina RAP-1 recombinant proteins are immunogenic for sheep. Thus, MSA-1 and RAP-1 are good candidates as antigens for the development of innovative diagnostic tests useful in the control of bovine babesiosis

Parasitology 2018 International Conference Keynote Speaker Abdalla Fadlalla Azrug Ahmed photo
Biography:

Abdalla Fadlalla Azrug has completed his PhD in Veterinary Helminthology at the age of 42 years at the Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, Turkey in 2011. He was the director for two regional veterinary research laboratories in West Sudan from 1999-2012. Recently he is the manager of the Central Veterinary Laboratory, Agriculture and Marine Resources Affairs, Manama, Bahrain, a governmental diagnostic and research laboratory in the veterinary services sector. He has published more than 15 papers in reputed journals and scientific international conference books participating in many international conferences related to the field of veterinary science and one health. He acted in the position of OIE delegate for Kingdom of Bahrain from 2014 -2017

Abstract:

Background:  Despite of Bahrain limited landspace, livestock production represents one of the back bones of the public economy. Goats and sheep have an important contribution in human livelihoods in Asia and Africa regions (FAO, 2015). Goats and sheep population mainly owned by smallholders in mixed farm systems. Gastrointestinal parasitism is one of the main health hazards associated with economic losses, lowered productivity and performance reduction. Material and Methods: A longitudinal 6 months study conducted in 7 sheep-goat farms located in the North Province of Bahrain following owners complains of emaciation, reduced productivity and losses among their herds during January – June 2017. The main objective of this investigative study was targeted for the prevalence of gastrointestinal parasites in such farms. The study conducted among 400 sheep and goats of different breeds, ages and sexes in closed and semi-closed farms. Fresh faecal and EDTA blood samples obtained directly from rectum and jugular veins respectively. A total of 73 (18.2%) samples and 41 (10.2%) blood samples collected from clinically infested animals. Also post-mortem gastrointestinal tract organs ( abomasum and rumen) collected from 6 freshly dead animals. Faecal egg counts (FEC per gram faeces) determined by Flotation and Sedimentation methods. Modified McMaster chamber technique used for identification and counting nematodes, cestodes and trematodes eggs and protozoan coccidia oocysts. Moreover, faecal cultures prepared by incubating 3-5-gram faeces at room temperature (24-27 C˚) for 7 -14 days to obtain infective larvae by modified Baerman apparatus technique. Blood smears from fresh EDTA blood samples for blood protozoan parasites in addition to determination of packed cell volumes (PCV) by hematocrit capillaries and centrifuging technique. Results: Haemonchus contortus was the most dominant helminth detected, 38 samples were positive (52.1%) of which (31.4%) were mixed with other helminth species. Other parasites detected involved Trichuris (17%), Moniezia (2.1%), Nematodirus (1.3%) and Eimeria (21.9%) respectively. Faecal egg counts per gram showed most cases of Heamonchosis with heavy infestation over (7-10)³ eggs /gram Parasitic prevalence rates estimated as percentage ratio of  number of positive samples divided by total number of animals examined ( d/n)%. Mature Haemonchus contortus were obtained by washing post-mortem abomasum into deep plastic trays, beakers and mature parasites collected using petri dishes with fine forceps examining under microscope. Most cases positive with Haemonchus contortus showed drop in  PCV indicating that Haemonchosis positive animals suffered from anemia. Discussions and Conclusion: Parasitic prevalence rates varied among ages where Hemochosis was mostly in old ages (3-5) years (58.7)% rather than young ages, while Eimeria mostly detected in young ages under 6 months (71.2)%. There was no significant effect noticed on animal sexes or breeds. Farm management systems, vegetation patterns and miss-usage of anthelmintics were the main risk factors associated with parasitic prevalence in Bahrain