Julio V Figueroa
CENID-PAVET, Mexico
Title: Immune recognition of Babesia bovis and Babesia bigemina MSA-1 and RAP-1 recombinant proteins in experimentally inoculated sheep
Biography
Biography: Julio V Figueroa
Abstract
Merozoite Surface Antigen-1 (MSA-1) and Ropthry Associated Protein-1 (RAP-1) for Babesia bovis and Babesia bigemina, respectively, have been involved in the invasion to the host erythrocyte. Moreover, considered as highly immunogenic proteins, they are candidate proteins to be used as specific antigens in Bovine babesiosis diagnosis. The main objective of this work was to determine the antibody recognition of Babesia bovis MSA-1 and RAP-1 antigens in serum of sheep immunized with the corresponding recombinant proteins, by using the Indirect Fluorescent Antibody Test (IFAT) and an indirect Enzyme-Linked Immunosorbent Assay (iELISA). The results of the study showed that recognition for the native antigen and the recombinant antigens of Babesia bovis and Babesia bigemina took place after the second immunization of animals as assessed by the two serological assays. The IFAT antibody titers for sheep inoculated with MSA-1 were 1:5120-1:10240, whereas for sheep inoculated with RAP-1 titers of 1:640 and 1:1280 were determined. A maximum absorbance reading of 0.689 and 0.600 were determined for MSA-1 and RAP-1, respectively, in the iELISA technique, concluding that the specific B. bovis MSA-1 and B. bigemina RAP-1 recombinant proteins are immunogenic for sheep. Thus, MSA-1 and RAP-1 are good candidates as antigens for the development of innovative diagnostic tests useful in the control of bovine babesiosis