Amany A Abd El-Aal
Cairo University, Egypt
Title: Local expression of T-cell population in sensitized and unsensitized experimental hydatidosis
Biography
Biography: Amany A Abd El-Aal
Abstract
Previous studies have focused mainly on the dominating Th2 cell response in chronic hydatidosis to benefit parasite growth and development. However, the status of the innate and adaptive immune cells and their contributions to E. granulosus cyst progression remains inadequately understood especially those related to sensitized hosts. The aim of the present experimental study was to investigate the local cellular patterns of T-cell population, T-helper 1 (Th1), T-helper 2 (Th2), T regulatory (Treg) and T cytotoxic (CD8) in sensitized (immunized) and unsensitized animals (control), infected with E. granulosus larval stages using specific immune-histochemical markers (STAT4, GATA3, FOXP3 and CD8) respectively. Significantly higher expression levels were recorded with STAT4 and CD8 in lesions related to sensitized group than control which showed nearly negative expression (95.07±9.51 and 59.73±2.91 vs. 1.36±0.49 and 0.64±0.32 respectively, P<0.0001). On the contrary GATA3 showed significantly lower values in sensitized vs. unsensitized control (39.83±2.01 and 77.94±6.63 respectively, P<0.0001). Therefore, killing of the larval cestodes was through inflammatory and cytotoxic effect related to Th1 & CD8 rather than B cell dependant pathway that is usually established through Th2. These results perhaps signify the reduction of the tolerance response induced by Th2 in immunized group. Therefore, our study strongly conceived the importance of the inflammatory and cytotoxic subset of T-cell population in the protective mechanism against hydatid infection following vaccination. Revealing the profile of these immune cells may help to develop new therapeutic and prophylactic strategies for this serious infection.