Parasitology

Biography

Biography: Manana Lortkipanidze

Abstract

This paper presents the results of using entomopathogenic nematodes for biological control of house fly Musca domestica L (Insecta: Diptera: Muscidae) in field conditions. The house fly, Musca domestica Linnaeus, is a well known cosmopolitan pest of both farm and home. This species is always found in association with humans or the activities of humans. The biological agents’ entomopathogenic nematodes of the Steinernematidae and Heterorhabditidae families are pathogenic for a range of pests. These nematodes are symbiotically associated with entomopathogenic bacteria Photorhabdus and Xenorhabdus. For the experiment we used pupae and larvae of fly (50-50) colonized 2 kg cattle dung. For infestation of insects the nematode suspension with certain concentration 10000 nematodes/ml was prepared. Three test samples were taken, to each dung sample was added 70, 50, 25 ml from the mentioned suspension. Appropriately, in test sample-I the number of nematodes was 350 per 1 g dung, in test sample-II: 250 and in test sample-III: 125. As the result showed in sample-I pupae and larvae mortality achieved 88.2-78%, in sample-II, mortality was 43.5-40% and in test sample-III, was approximately 32.3-28.3%. The insects died mostly in the pupa stage. The analysis of the experiments conducted by us provide evidence that the most efficient dose of the nematode suspension applied against pupae and larvae of fly colonized on cattle dung is 350 IJs/g. Both species of entomopathogenic namatodes produced mortality of experimental insects, although the S. feltiae was more significant than H. bacteriophira.