Ankita Thakur
Panjab University, India
Title: Protective immunity using different adjuvants in combination with Killed Leishmania donovani antigen against experimental visceral leishmaniasis
Biography
Biography: Ankita Thakur
Abstract
Visceral leishmaniasis (VL) is a neglected tropical disease caused by protozoan parasites of the Leishmania donovani complex. An estimated 200 000 to 400 000 new cases are reported annually. Control of VL infection till date relies on chemotherapy. The therapeutic armamentarium for VL is currently plagued with several limitations as the available drugs are toxic, expensive and need to be administered for extended periods. So there is an urgent need to find another alternative in the form of a vaccine. Killed vaccines composed of parasite fractions or whole killed parasites reached phase 3 clinical trials, but showed a limited prophylactic efficacy. It seems that major limiting factor for the development of killed vaccines is lack of a suitable adjuvant. Therefore, the aim of the present study was to enhance the protective efficacy of Killed Leishmania donovani (KLD) antigen by using different adjuvants. Inbred BALB/c mice were immunized with KLD antigen alone and in combination with four different adjuvants i.e. alum, saponin, MPL-A and cationic liposomes. Three immunizations were carried out at an interval of two weeks. Two weeks after last booster challenge infection was given and mice were sacrificed on different post challenge days. Protective efficacy of vaccines was analyzed by assessment of parasite burden and generation of cellular and humoral immune responses. All the formulations were found to be immunogenic and imparted significant protection. However, level of protection varied with the type of adjuvant used. Our findings suggest promising antileishmanial potential of KLD as an antigen candidate in combination with cationic liposomes and MPL-A as adjuvants against murine visceral leishmaniasis.