Ying Liu
Henan Centre for Disease Control and Prevention, China
Title: Circumsporozoite Protein Gene Diversity of Plasmodium vivax isolates from Henan Province,China
Biography
Biography: Ying Liu
Abstract
Plasmodium vivax（P.v） malaria is one of major parasitic diseases with a long history in Henan, China, but little is known about the genotypic polymorphism of P.v in this area. This study aimed to investigate the genetic diversity of circumsporozoite protein (CSP) gene in P.v from Henan, China. Thirty-two P.v isolates from Henan province were characterized by analysing the genetic polymorphism of the CSP gene, all the isolates were found to belong to the VK210 type. The isolates tested displayed variations in the peptide repeat motifs with different size（20-22）or different order, eight different sub-types were observed in the isolates analysed. Compared with the VK210 basic repeat units (GDRADGQPA), more than 75% repeat units have base transition non silence at least one time. seven types of repeat elements were observed in the central repeat units, the change mainly occurred in 3, 4, 6, 7, 9, 14, 21, 22 nucleotides, among which,3,21 often silent while 4,7,9,14,22 often non silent, and the sixth nucleotide can occur base transform both silent and non silent base transform. The nucleotide sequences of Henan isolates were compared with the published sequences, from the genetic distance, isolates from Henan were further with those from Africa and Southeast Asia. The results obtained in the present study indicate that the P. v parasite population is highly diverse in Henan province, and the genotyping protocols used in this study may be useful for differentiating the imported cases from the indigenous ones in vivax malaria.