3rd World Conference on Parasitology & Pathogenesis
Chicago, USA
Santi P. Sinha Babu
Visva-Bharati University, India
Title: A novel ligand of toll-like receptor 4 from Wuchereria bancrofti induces classical macrophage activation
Biography
Biography: Santi P. Sinha Babu
Abstract
Lymphatic filariasis (LF), a vector-borne parasitic disease, is endemic in several parts of the globe and causes morbidities, disabilities and socio-economic loss to a large population every year. Inflammatory events resulted from host-parasite interactions play key role in the pathology of LF. The study was undertaken with an objective to isolate the proinflammatory factor from microfilaria of Wuchereria bancrofti. A~70 kDa microfilarial protein (MfP) was isolated from the lysate of W. bancrofti microfilariae through ultrafiltration followed by p-amino phenyl phosphoryl choline affinity chromatography. Inflammation of MΦ was studied by determining the expression of TLR4, its downstream signaling molecules and proinflammatory cytokines using immunoblotting and PCR. In this investigation, we have found that MfP of W. bancrofti binds to macrophage (MΦ)-TLR4 receptor. This protein triggers TLR4 signaling pathway through NF-κB activation. It was also evident from elevated secretion of proinflammatory cytokines i.e., TNF-α, IL-6 and IL-1β. To examine the specificity of MfP for augmenting TLR4 mediated proinflammatory signals, TLR4 KO MΦ were incubated with MfP, failure of MfP to evoke proinflammatory responses through NF-κB activation suggested MfP induced mediation of TLR4 activity. Additionally, fluorescent labeled MfP demonstrated its binding to TLR4. MfP therefore appears to be a new ligand on the surface of W. bancrofti, determination of its functional attributions in host-parasite relationship may open an unknown area in our understanding.